Construction of two Plasmids, Each Containing Three Different Human Globin Gene Fragments

Abstract

Two plasmids (labelled pUC-2γ-B and pUC-2β-δ), each containing three different human globin gene fragments (γIVS-II, inter-γ, and βIVS-II in pUC-2γ-β, and 5'-β, 3'-β, and δIVS-II in pUC-2β-δ) have been constructed. The three gene fragments could be isolated simultaneously from Eco RI/Bam HI double digests of each of the two plasmids using electrophoresis in an 1.0% agarose gel. This facilitates the large-scale isolation of the gene fragments for use as probes in studies such as the analysis of β-globin gene cluster polymorphisms or of the molecular basis of some hemoglobinopathies.