Abstract
The minor components of Hb Bart's were separated by CM-cellulose chromatography, reverse-phase HPLC, and DEAE-cellulose chromatography. These were characterized by amino acid analysis, tryptic peptide analysis by HPLC, electrophoresis, and carbohydrate and phosphate analysis. Acetylated and glycated components of Hb Bart's were present in cord blood red cells. The ratio of γTC/total γ in Hb Bart's was nearly the same as that of HbIC/Hb Fwhich may indicate that the γ chain after its release from the ribosome is not a better acetylatlon substrate than Hb FO. Glycation of the free γ-chains seems to take place in vivo as readily as the major Hb components.