Publication Cover
Hemoglobin
international journal for hemoglobin research
Volume 12, 1988 - Issue 5-6
2
Views
0
CrossRef citations to date
0
Altmetric
Original Article

Construction of Three Plasmids, Each Containing two or three Different Human Globin Gene Fragments

&
Pages 755-761 | Received 22 Dec 1987, Accepted 01 Jun 1988, Published online: 07 Jul 2009
 

Abstract

Three plasmids (labeled pUC-2γ-β, pUC-2β-δ, and pL-α-ζ), each containing two or three different human globin gene fragments (βIVS-II, inter-γ, and γIVS-II in pUC-2γ-β; 5′-β, 3′-β, and δIVS-II in pUC-2β-δ; α and ζ in pL-α-ζ) have been constructed. The gene fragments could be isolated simultaneously from Eco RI/Bam HI double digests of each of the first two plasmids or from a Pst I digest of the third, using electrophoresis in an 1.0% agarose gel. This facilitates the large-scale isolation of gene fragments for use as probes in studies such as the analysis of polymorphisms or the molecular basis of some hemoglobinopathies.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.