Construction of Three Plasmids, Each Containing two or three Different Human Globin Gene Fragments

Abstract

Three plasmids (labeled pUC-2γ-β, pUC-2β-δ, and pL-α-ζ), each containing two or three different human globin gene fragments (βIVS-II, inter-γ, and γIVS-II in pUC-2γ-β; 5′-β, 3′-β, and δIVS-II in pUC-2β-δ; α and ζ in pL-α-ζ) have been constructed. The gene fragments could be isolated simultaneously from Eco RI/Bam HI double digests of each of the first two plasmids or from a Pst I digest of the third, using electrophoresis in an 1.0% agarose gel. This facilitates the large-scale isolation of gene fragments for use as probes in studies such as the analysis of polymorphisms or the molecular basis of some hemoglobinopathies.