Hb Ullevaal [β78(EF2)Leu→Val; HBB: c.235C>G], a New Hemoglobin Variant Interfering with Hb A_1c Measurement Using a Cation Exchange High Performance Liquid Chromatography Method

Runa M. GrimholtDepartment of Medical Biochemistry, Oslo University Hospital Ullevaal, OsloNorwayCorrespondence[email protected]

Åshild A. SudmannDepartment of Medical Biochemistry, Oslo University Hospital Ullevaal, OsloNorway

Armin P. PiehlerFürst Medical Laboratory OsloNorway

Petter UrdalDepartment of Medical Biochemistry, Oslo University Hospital Ullevaal, OsloNorway

Olav KlingenbergDepartment of Medical Biochemistry, Oslo University Hospital Rikshospitalet, OsloNorway

Abstract

A new hemoglobin (Hb) variant was detected during Hb A_1c measurement. DNA sequencing showed heterozygosity for the single nucleotide substitution (C > G) on the β-globin gene causing an amino acid change [β78(EF2)Leu→Val; HBB: c.235C > G]. The new Hb variant was designated Hb Ullevaal after the hospital at which it was discovered. Heterozygosity for Hb Ullevaal appears to have no clinical significance. However, it interferes with Hb A_1c measurement by cation exchange high performance liquid chromatography (HPLC), causing falsely low Hb A_1c concentration when using the Tosoh G7 apparatus in variant mode.

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Hb Ullevaal [β78(EF2)Leu→Val; HBB: c.235C>G], a New Hemoglobin Variant Interfering with Hb A_1c Measurement Using a Cation Exchange High Performance Liquid Chromatography Method

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Hb Ullevaal [β78(EF2)Leu→Val; HBB: c.235C>G], a New Hemoglobin Variant Interfering with Hb A1c Measurement Using a Cation Exchange High Performance Liquid Chromatography Method

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Hb Ullevaal [β78(EF2)Leu→Val; HBB: c.235C>G], a New Hemoglobin Variant Interfering with Hb A_1c Measurement Using a Cation Exchange High Performance Liquid Chromatography Method