Publication Cover
Hemoglobin
international journal for hemoglobin research
Volume 14, 1990 - Issue 5
9
Views
30
CrossRef citations to date
0
Altmetric
Original Article

New Results of Hemoglobin Variant Structure Determinats by Fast Atom Bombardment Mass Spectrometry

, , , , , , & show all
Pages 529-548 | Received 12 Mar 1990, Accepted 11 Sep 1990, Published online: 07 Jul 2009
 

Abstract

Fast atom bombardment mass spectrometry has already been used for the identification of mutations in abnormal human hemoglobin chains. This paper presents new results obtained with this technique. The methodology used here is compared with more conventional biochemical techniques and automated microsequencing. In every case, a well-chosen combination of peptide-high performance liquid chromatography, mass spectrometry, amino acid analysis, and sequence analysis led rapidly to the identification of the mutant. The high sensitivity of these techniques holds great promise for the analysis of molecular abnormalities in various genetic disorders presently detectable only by the application of a molecular biological approach.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.