Detection of the HB Quong SZE Mutation in a Chinese Family by Selective Amplification of the α2-Globin Gene and Restriction Map Analysis with MSP I

Abstract

The polymerase chain reaction technique combined with restriction map analysis with Msp I and hybridization with synthetic oligonucleotide probes has been used to identify Hb Quong Sze [α25(H8)Leu→Pro] in a Chinese family in Guangxi (Quong Sze), P.R. China. Our data and those described in an earlier publication (1) indicate that the Hb Quong Sze carriers originate from the same province of the People's Republic of China, namely Guangxi.