The Use of Staphylococcus V8 Protease in the Structural Determination of Hunan Hemoglobin Variants:Hb Valparaiso [α88(F9)ALA-GLY] As Example

H. Wajcman INSERM U299, Hospital de Bicetre, 94275, Le Kremlin-Bicětre, France

M. de Montalembert Centre de Transfusion Sanguine, Hospital Necker-Enfants Malades, 75015, Paris, France

R. Girot Laboratoire d'Hématologie, Hospital Tenon, 75020, Paris, France

J. Kister INSERM U299, Hospital de Bicetre, 94275, Le Kremlin-Bicětre, France

M. Maier-Redelsperger Laboratoire d'Hématologie, Hospital Tenon, 75020, Paris, France

F. Galacteros INSERM U91, Hospital Henri Mondor, 94010, Créteil, France

Abstract

In human hemoglobin, enzymic cleavage with the protease from Staphylococcus aureus strain V8 (protease V8) is a convenient method for the study of variants that involve large peptides such as αT-9 and αT-12b. We report here on Hb Valparaiso [α88(F9)Ala-≫Gly], a new neutral variant with a slight increase in oxygen affinity, that was identified by use of this strategy.

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The Use of Staphylococcus V8 Protease in the Structural Determination of Hunan Hemoglobin Variants:Hb Valparaiso [α88(F9)ALA-GLY] As Example

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The Use of Staphylococcus V8 Protease in the Structural Determination of Hunan Hemoglobin Variants:Hb Valparaiso [α88(F9)ALA-GLY] As Example

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