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Hemoglobin
international journal for hemoglobin research
Volume 20, 1996 - Issue 4
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Original Article

Gelation Kinetics of Dilute Hemogln from Sickle Cell Anemia Patients

Pages 415-428 | Received 04 Aug 1995, Accepted 22 May 1996, Published online: 07 Jul 2009
 

Abstract

Non-ideal behavior of sickle cell hemoglobin (Hb S) and varying experimental conditions have made gelling kinetic studies difficult. A new gelation kinetic procedure is developed in this study. This technique uses low Hb S concentrations (20-fold less than in conventional methods) without the need for a temperature jump. Gelation progress, monitored with a newly developed turbidimetric procedure at 815 nm, was fitted to a mono-exponential and a sigmoid-Emax models. These models allowed precise definitions of gelation delay periods, rate of rapid Hb S gelation and a parameter, T1/2 which combines information from the delay and rapid gelation stages. All these parameters vary linearly with Hb S concentration. The merits of using T1/2 are discussed.

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