Globin Chain Synthesis Analysis by High Performance Liquid Chroniatography in the Screening of Thalassemia Syndromes

Abstract

We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for β-thalassemia. The α/non-α-globin chain synthesis ratios have been determined in α-, β-, and δβ-thalassemia carriers using the classical carboxymethyl cellulose chromatography as the reference method. Reversed phase high performance liquid chromatography is fast, accurate, and reproducible, and may be a suitable alternative for the traditional carboxymethyl cellulose chromatography.