In Vitro Interaction of Sized and Unsized Liposome Vesicles with High Density Lipo Proteins

Abstract

An in vitro method of interaction of liposome vesicles with high density lipoproteins (HDL) was studied. A formulation of lipids was hydrated, and homogenized with a high shear mixer and designed as “unsized” liposomes. A portion of this preparation was further processed by passing through a polycarbonate membrane and it is labelled as “sized”. Both these preparations were incubated with HDL. Destabilization rate of liposome preparations in presence of HDL was determined. Time course of drug release from sized and unsized liposomes was followed upon HDL addition to liposomes. These in vitro studies clearly showed that HDL destabilized the small sized liposomes (0.25 ± 0.09 Hm). The destabilization effect of HDL on unsized is not significant.