Abstract
A reversed-phase ion-pair high performance liquid chromatographic assay, which can simultaneously determine norfloxacin and its decomposition products, formyl piperazine and ethylenediamine analogs in aqueous media, has been developed. This assay has been applied to a stability study of norfloxacin in aqueous media. The effects of temperature, pH, oxygen and light on norfloxacin have been investigated using a 23×3 factorial design. Results indicate that oxygen, light, temperature and pH have significant effect on the stability of norfloxacin solution. Norfloxacin is most stable at acidic and basic pH, in darkness, in the absence of oxygen and at low temperature.