Simultaneous Quantitative Determination of Tinidazole and Diloxanide Furoate in Tablet Preparations by Difference Spectroscopy

Abstract

A difference apectrophotoaetric procedure has been developed for the simultaneous determination of Tinidazole (TD) and Diloxanide furoate (DF) in tablet preparations. The method comprised the measurement of absorbance of a solution of the tablet extract in pH 2.0 buffer solution relative to that of an equimolar solution in pH 13.0 buffer at the wavelengths of 282nm and 240nm. The presence of identical isoabestic points for pure drug samples and tablet extract solutions indicated tho non-interference of excipients in the absorption at these wavelengths. The compliance of Beer's Law was obtained in the concentration range of 20–40μg/ml for TD and DF at theee wavelengths.