Cryoprotection of Emulsions in Freeze-Drying: Freezing Process Analysis

J. Bamba Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, URA CNRS, 1218 UniversitC Paris Sud, 92296, Chltenay-Malabry, Cedex, France

G. Cavé Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, URA CNRS, 1218 UniversitC Paris Sud, 92296, Chltenay-Malabry, Cedex, France

Y. Bensouda Laboratoire de Pharmacie Galénique, Faculté de Médecine et Pharmacie, Rabat, Maroc

P. Tchoreloff Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, URA CNRS, 1218 UniversitC Paris Sud, 92296, Chltenay-Malabry, Cedex, France

F. Puisieux Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, URA CNRS, 1218 UniversitC Paris Sud, 92296, Chltenay-Malabry, Cedex, France

G. Couarraze Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, URA CNRS, 1218 UniversitC Paris Sud, 92296, Chltenay-Malabry, Cedex, France

Abstract

We report the effects of different freeze/thawing processes on oil-in-water emulsions. The influence of the oil phase concentration, the cooling rate and the addition of carbohydrates as cryoprotectors were investigated. It appears that a slow cooling rate and the addition of cryoprotectants which do not crystallize during freeze/thawing have the best stabilizing effects. These stabilizing effects were attributed to the amorphous state of the cryoprotectants and the lower mechanical stress due to the slow cooling rate. Heat treatment before thawing causes crystallization and decreases the stabilizing effects.

These results show the importance of crystalline state of additives used as cryoprotectants during freeze/thawing.

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Cryoprotection of Emulsions in Freeze-Drying: Freezing Process Analysis

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Cryoprotection of Emulsions in Freeze-Drying: Freezing Process Analysis

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