Determination of Flavopiridol (L 86 8275) in Rat Serum Using Ion-Pair Reversed-Phase High-Performance Liquid Chromatography

Abstract

A specific, sensitive, and simple high-performance liquid chromatographic method has been developed for assaying flavopiridol in rat serum for preliminary bioavailability and pharmacokinetic studies. The drug and internal standard were extracted with dichloromethane from serum. After evaporating the solvent, the extract was reconstituted with mobile phase. Chromatography was performed on a 10-μ. Bondapak C₁₈ column (30 cm × 3.9 mm ID), using methanol:water (50:50, v/v) containing 0.05 M ion-pairing reagent PIC B-6 as a mobile phase at a flow rate of 1.1 ml/min, with ultraviolet detection at 278 nm. The drug and internal standard were well resolved from other serum constituents. Due to high sensitivity and reproducibility the method can be adapted very well for assaying flavopiridol in higher animals.