High Performance Liquid Chromatographic Determination of Sodium Cromoglycate

Abstract

A simple and precise liquid chromatographic method was developed for the estimation of sodium cromoglycate (SC) in pharmaceuticals. The drug was chroma-tographed on a reverse phase C18 column. The eluants were monitored at a wavelength of 325 nm utilizing a mixture (80:20) of solution A: 0.025 M 1-octane sulfonic acid sodium salt in acetic acid (1:100), and solution B: methanol, aceto-nitrile (3:2). Solution concentrations were measured on a weight basis to avoid the use of an internal standard. The method was statistically validated for its linearity, accuracy, precision, selectivity, limit of detection and limit of quantitation. Due to its simplicity and accuracy, the authors believe that the method can be used for routine quality control analysis. It does not require any specific sample preparation except for the use of a column guard before the analytical column and a suitable profiler attached to the syringe prior to injection.