Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems

ABSTRACT

The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G₁ phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.

Keywords: :

• Cell cycle
• CGH
• Cytogenetic analysis
• Spheroids
• Colon carcinoma

ACKNOWLEDGMENTS

The authors thank Simona Ramponi and Vito Lorusso, Bracco SpA for SEM images and Sergio Bernasconi and Chiara Mariani for technical support.