Antiproliferative Activity of Larrea Divaricata Cav. on Lymphoma Cell Line: Participation of Hydrogen Peroxide in Its Action

ABSTRACT

Larrea divaricata is a plant used in Argentina. The aim was to analyze the participation of H₂O₂ in the antiproliferative effect of L divaricata on BW5147 cells. Different studies were performed: proliferation, viability, nitrite production, apoptosis, oxygen reducing activity, NF-κ B translocation, H₂O₂ production, H₂O₂ scavenging activity, and effect on exogenous superoxide dismutase. The extract decreased proliferation and induced apoptosis through P-38 and ERK pathways. The extract activated Mn^{2 +}–SOD, increasing H₂O₂, which was implicated in the antiproliferative mechanism by the increase of Nitric oxide (NO). New drugs, which can increase H₂O₂, could be a therapeutic strategy for the treatment of cancer.

Keywords: :

• Lymphocytes
• Proliferation
• Hydrogen peroxide

ACKNOWLEDGMENTS

This work was supported by funds provided by PIP CONICET 5232. Roberto Davicino is a postdoctoral fellow of CONICET.