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ABSTRACT
Objectives: To explore the role of SEPT7 in glioma cell invasion. Methods: SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin αvβ3, and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of α-tubulin was examined by laser scanning confocal analysis. Result: After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin αvβ3 was decreased, while TIMP1/2 was increased, and α-tubulin was redistributed. Conclusion: These results suggest that SEPT7 plays an important role in the glioma cell invasion.
ACKNOWLEDGMENTS
This work is supported by National Natural Science Foundation of China, grant numbers 30500523 and 30872985; Tianjin Science and Technology Committee, grant number 06YFSZSF01100; Tianjin Education Committee, grant number 20070234; and Program for New Century Excellent Talents in University (NCET-07-0615). We thank Eline Menu for the assistance with language corrections.