Can hsp90α-Targeted siRNA Combined With TMZ Be a Future Therapy for Glioma?

ABSTRACT

Hsp90α's vital role in cell cycle progression and apoptosis together with its presence in gliomas and absence in normal tissue, make it a credible target for cancer therapy. Three sets of dsRNA oligos designed to align different regions of the hsp90α sequence were used to downregulate hsp90α. SiRNA 1, 2, and 3 resulted in significant levels of silencing of hsp90α after 48 hr treatment (p < .0001). Concurrent treatment of the glioma cell line U87-MG with siRNA 1 and temozolomide (TMZ) resulted in a 13-fold reduction in the dose of TMZ required to achieve a similar effect if TMZ was used alone.

Keywords :

• Glioma
• Normal brain
• Hsp90α
• siRNA