SMARCA5 Methylation and Expression in Gastric Cancer

Carolina Oliveira GigekDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil,

Luara Carolina Frias LisboaDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil,

Mariana Ferreira LealDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil,

Patricia Natalia Oliveira SilvaDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil,

Eleonidas Moura LimaDepartamento de Biologia, Campus Ministro Reis Velloso/Parnaíba, Universidade Federal do Piauí, Teresina, Piaui, Brazil,

André Salim KhayatLaboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, PA, Brasil

Paulo Pimentel AssumpçãoServiço de Cirurgia, Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, PA, Brasil

Rommel Rodriguez BurbanoLaboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, PA, Brasil

Marilia de Arruda Cardoso SmithDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil,Correspondence[email protected]

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Abstract

Here, we first evaluated SMARCA5 expression and promoter DNA methylation in gastric carcinogenesis. Immunohistochemistry and methylation-specific PCR were analyzed in 19 and 48 normal mucosa and in 52 and 92 gastric cancer samples, respectively. We observed higher immunoreactivity of SMARCA5 in gastric cancer samples than in normal mucosa. Moreover, SMARCA5 promoter methylation was associated with the absence of protein expression. Our findings suggest that SMARCA5 has a potential role in proliferation and malignancy in gastric carcinogenesis.

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ACKNOWLEDGMENTS

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). R. R. Burbano and M. A. C. Smith have research grants from CNPq. C. O. Gigek and M. F. Leal have fellowships granted by FAPESP.

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SMARCA5 Methylation and Expression in Gastric Cancer

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SMARCA5 Methylation and Expression in Gastric Cancer

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