Association of Functionally Important Polymorphism of Microsomal Epoxide Hydrolase Gene (EPHX1) With Lung Cancer Susceptibility

Abstract

Distribution and gene–environment interaction of EPHX1 polymorphism was evaluated in 175 lung cancer patients and 322 controls from north India. Two novel non-synonymous, Lys117Arg and Leu263Phe, and twelve single nucleotide polymorphisms were identified in the present study. Binary logistic regression analysis showed association of polymorphism Tyr113His with increased risk of lung cancer (OR = 2.2, 95% CI = 1.2–4.0, p < .05). Gene–environment interaction revealed that patients with His113His and smoking habit had significantly greater risk of lung cancer (OR = 4.52, 95% CI = 0.93–43.05, p < .05). Present study provided evidence that EPHX1 polymorphism is associated with lung cancer susceptibility in Indian population.

Keywords: :

• Lung cancer
• EPHX1
• Single nucleotide polymorphism (SNP)

ACKNOWLEDGMENT

Anup Raj Tilak is grateful to CSIR, India, for providing the fellowships during this work. The financial support of CSIR-network project CMM-003 (Toxicogenomics) in carrying out this study is gratefully acknowledged. We thank Dr. Ved Prakash Verma, Department of Radiotherapy, CSJM Medical University, Lucknow (India), for help in sample collection.

Table S1 Primer Sets Used for the Amplification of Different Exons of EPHX1 Gene