Distinguishing Non-Small Cell Lung Adenocarcinoma Patients from Squamous Cell Carcinoma Patients and Control Individuals Using Serum Profiling

Abstract

Goals of this study were to analyze the ability of mass spectrometry serum profiling to distinguish non-small cell lung adenocarcinoma from squamous cell carcinoma patients and healthy controls. Sera were obtained from 19 adenocarcinoma patients, 24 squamous cell carcinoma patients, and 21 controls. Identifications of significant mass-to-charge ratio (m/z) peak differences between these groups were performed using t-tests. A “leave one out” cross-validation procedure yielded discriminatory lung adenocarcinoma versus squamous cell carcinoma p and ROC curve values of <.0001 and 0.92, respectively. Test sensitivity and specificity were 84% and 79%, respectively. This approach could aid in lung cancer diagnosis and sub-typing.

Keywords: :

• Non-small cell lung cancer
• Adenocarcinoma
• Squamous cell carcinoma
• Serum profiling
• Early detection
• Predictive value analysis
• Electrospray mass spectrometry

ACKNOWLEDGMENTS

This work was supported by grants HR03–043 and AR08–016 from the Oklahoma Center for the Advancement of Science & Technology, and grant C1063804 from the Oklahoma Tobacco Research Center.