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CELLULAR AND MOLECULAR BIOLOGY

IGF-1 Deficiency in Combination with a Low Basic hBD-2 and hBD-3 Gene Expression Might Counteract Malignant Transformation in Pleomorphic Adenomas in Vitro

, , , , , , , , , & show all
Pages 106-113 | Published online: 02 Feb 2012
 

Abstract

This study investigated the IGF-1-influence on oncological relevant genes in pleomorphic adenomas.

Therefore A64-tumorcells were stimulated by recombinant IGF-1. After RNA-extraction, transcript levels of hBD-1, hBD-2, hBD-3, DEFA1/3, DEFA4, S100A4, Psoriasin, DOC-1, EGF, EGFR, and IGFR were analyzed by qRT-PCR at t = 0, 4, 8, 24, 48, and 72 hr. The gene-products were visualized by immunostaining.

A64-tumor-cells were deficient for hBD-1 and IGF-1. IGF-1 downregulates hBD-2 and hBD-3 without influencing hBD-1-expression. IGF-1 only slightly affects DEFA1/3-, DEFA4-, S100A4-, Psoriasin-, DOC-1-, EGF-, EGFR-, and IGFR-gene-expression.

IGF-1-deficiency combined with low basic hBD-2-gene-expression and hBD-3-gene-expression might counteract, whereas hBD-1-deficiency promotes malignant transformation in pleomorphic adenomas.

ACKNOWLEDGMENT

This study was supported by the BONFOR research foundation of the Medical Faculty of the University of Bonn. J. Winter (TP 10), M. Wenghoefer (TP 10), S. Jepsen (TP 2), JP. Allam (TP 1), N. Novak (TP 1), and W. Götz (TP 7) were supported by Deutsche Forschungsgemeinschaft (KFO 208).

DECLARATION OF INTERESTS

The authors declare that they have no competing interests.

ABBREVIATIONS
DMEM=

 Dulbecco's Modified Eagle's Medium

Doc-1=

 Deleted in oral cancer-1

EGF(-R)=

 Epidermal growth factor (receptor)

HER-2=

 Human epidermal growth factor receptor 2

hBD=

 human beta defensin

IGF(-R)=

 Insulin-like growth factor (receptor)

n.d.=

 not detectable

OSCC=

 Oral squamous cell carcinoma

qRT-PCR=

 Quantitative realtime polymerase chain reaction

Rel.=

 Relative

SD=

 Standard deviation

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