Rapid sensitive analysis of IDH1 mutation in lower-grade gliomas by automated genetic typing involving a quenching probe

ABSTRACT

The authors recently found that 80% of lower-grade gliomas (LGGs) harbored a mutation in IDH1. Intraoperative detection of the mutated IDH1 helps not only differentiate LGGs from other type of brain tumors, but determine the resection border. In the current study, the authors have applied an automated genetic typing involving a quenching probe to detect the mutated IDH1. If tumor cells with the mutated IDH1 contained 10% or more in the mixture of normal and tumor cells, the device could detect it sensitively. The intraoperative assessment of IDH1 mutation is useful in brain tumor surgeries.

Keywords:

• Brain tumors
• Cancer biomarkers
• Gene profiling

Acknowledgments

The authors thank Mr. Yuki Koyamoto, Mr. Junichi Kato, and Mr. Isamu Nakamura (Arkray) for their technical assistance. This work was supported by a Grant-in Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (A.N.; 23107010).

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.