6
Views
4
CrossRef citations to date
0
Altmetric
Original Article

Effect of Plasma Fibronectin, Macrophages, and Glycosaminoglycans on Tumor Cell Growth

, &
Pages 339-345 | Published online: 11 Jun 2009
 

Abstract

Fibronectin (Fn) synergizes with macrophages (MϕS) in inducing cytostasis and cytotoxicity of neoplastic cells in culture. Since heparin enhances Fn's opsonic activity in many systems, we investigated its effect on Fn-macrophage synergy in cytostasis. MCG-T14 (a spontaneous mouse mammary adenocarcinoma) cells (4 × 104) were added to wells both with and without C. parvum activated MϕS monolayers. To these cultures were added increasing concentrations of Fn with or without heparin. Fn synergizes with both MϕSs and heparin in inhibiting tumor cell growth. The combined cytostatic effect of Fn, heparin and MϕSs is more than additive. In other experiments, MCG-T14 cells were pre-incubated for 2 hr with Fn, washed free of Fn, and treated as above. The results of these experiments were similar to coculture experiments, but the effect of heparin was even more pronounced. Dermatan sulfate and hyaluronic acid had a variable effect on Fn and Fn-macrophage induced cytostasis.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.