New Developments in Protein Isolation, Purification, and Characterization

Abstract

The most significant advancements in techniques and methods for protein purification and analysis have been made in liquid chromatography and in electrophoresis. In the area of chromatography, adsorbents based on new affinity principles have been prepared. New packing materials have facilitated the rapid progress of high-performance techniques. A great many new techniques in the field of electrophoresis have emerged. On an analytical scale, electrophoretic methods in two dimensions or in capillaries are unsurpassed in resolution power. Development of techniques for protein transfer between different media is a prerequisite for a full exploitation of the new methods. Modern techniques for analysis of submicrogram quantities facilitate the separation, detection, and characterization of complex protein mixtures.