³H-NECA Binding to Polymorphonuclear Membrane: Effect of Sera from Patients with Hodgkin's Disease

Abstract

Several studies have shown that seru from patients with Hodgkin's disease contain factors capable of inhibiting polymorphonuclear functions, among them chemotaxis. In the present study we investigated whether these sera, which were able to inhibit PMN chemotaxis in the agurose test, were also able to affect the ³H-NECA binding to PMN membrane obtained from healthy donors. Control experiments were carried out using PMN incubated with a pool of sera from healthy volunteers.

No significant difference was found in the maximum number of binding sites; on the contrary, the equilibrium dissociation constant was significantly increased in the membrane prepuration of PMN incubated with pathological serum.