Effects of Forward and Backward Transitions in Light Intensities in Tau-Illuminance Curves of the Rat Motor Activity Rhythm Under Constant Dim Light

Abstract

Circadian rhythms are strongly influenced by light intensity, the effects of which may persist beyond the duration of light exposure (aftereffects). Here, the authors constructed period-illuminance curves for the motor activity circadian rhythm of male and female rats by recording the effects of a series of small upward and downward steps in light intensity (illuminance ranging between .01 lux of dim red light and 1 lux of white light) on their activity. In all cases, stepwise changes were made in five logarithmic steps (irradiance: dim red light: .692 µW/cm² and white light: .006, .016, .044, .12, and .315 µW/cm², corresponding, respectively, to .02, .05, .14, .13, and 1 lux measured at cage level), with changes in intensity every 2 wks. One group of rats (DLD) started in dim red light, moved up to 1 lux white light, and then back down to the original light intensity. Another group (LDL) started at 1 lux, moved down to .01 lux, and then back up to the original intensity. Motor activity data were recorded throughout the experiment and tau values, the percentage of variance explained by the rhythm, and the mean motor activity for each stage and group were calculated. The results show differences in the dynamics of tau values between the DLD and LDL groups and between males and females. In the LDL group, the tau values of both males and females were dependent on light intensity, and were similar for the forward and backward transitions. In other words, no aftereffects were found, and no differences were detected between males and females. In the DLD group, however, differences were found between males and females. Males had a tau value of 24 h 20 min under dim red light, 25 h 40 min under 1 lux, and 24 h 50 min on return to dim red light. It is noticeable that the tau values of the backward branch of the illuminance curve contradicted classical predictions, since at .38 and .14 lux the tau values were shorter than those found under the same intensities after exposure to 1 lux. Females became arrhythmic at 1 lux, and only one half of them recovered their circadian rhythm at .02 lux. The other one half remained arrhythmic even under dim red light. Thus, some of the results of this paper contradict the predictions of standard descriptions of the functioning of the circadian clock, possibly due to the effects of dim light. (Author correspondence: [email protected])

Keywords:

• Circadian rhythm
• Light intensity
• Motor activity
• Rats
• Tau-illuminance curves

ACKNOWLEDGMENTS

This work was supported by the Ministerio de Educación y Ciencia (project: BFU2008-00199).

Declaration of Interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.