Atypical expression of circadian clock genes in denervated mouse skeletal muscle

Abstract

The central circadian clock in the suprachiasmatic nucleus of the hypothalamus synchronizes peripheral clocks through neural and humoral signals in most mammalian tissues. Here, we analyzed the effects of unilateral sciatic denervation on the expression of circadian clock- and clock-controlled genes in the gastrocnemius muscles of mice twice per day on days 0, 3, 7, 9, 11 and 14 after denervation and six times on each of days 7 and 28 after denervation to assess the regulation mechanism of the circadian clock in skeletal muscle. Sciatic denervation did not affect systemic circadian rhythms since core body temperature (Day 7), corticosterone secretion (Days 7 and 28), and hepatic clock gene expression remained intact (Days 7 and 28). Expression levels of most circadian clock-related genes such as Arntl, Per1, Rora, Nr1d1 and Dbp were reduced in accordance with the extent of muscle atrophy, although circadian Per2 expression was significantly augmented (Day 28). Cosinor analysis revealed that the circadian expression of Arntl (Days 7 and 28) and Dbp (Day 28) was phase advanced in denervated muscle. The mRNA expression of Clock was significantly increased in denervated muscle on Day 3 when the severe atrophy was absent, and it was not affected by atrophic progression for 28 days. Sciatic denervation did not affect the expression of these genes in the contralateral muscle (Days 7 and 28), suggesting that humoral changes were not involved in denervation-induced muscle clock disruption. We then analyzed genome-wide gene expression using microarrays to determine the effects of disrupting the molecular clock in muscle on circadian rhythms at Day 7. Among 478 circadian genes, 313 lost rhythmicity in the denervated muscles. These denervation-sensitive genes included the lipid metabolism-related genes, Nrip1, Bbs1, Ptgis, Acot1, Scd2, Hpgd, Insig1, Dhcr24, Ldlr and Mboat1. Our findings revealed that sciatic denervation disrupts the circadian expression of clock and clock-controlled genes either directly or indirectly via muscle atrophy in the gastrocnemius muscles of mice in a gene-specific manner.

• Circadian rhythm
• DNA microarray
• gastrocnemius muscle
• muscle atrophy
• peripheral clock
• sciatic denervation
• soleus muscle

ACKNOWLEDGEMENTS

We thank Dr. Koyomi Miyazaki (AIST), Dr. Tatsunosuke Tomita (AIST), Dr. Hideaki Oike (National Agriculture and Food Research Organization), Dr. Toshiko Ohta (University of Tsukuba) and Dr. Hiroshi Ohshima (JAXA) for valuable comments. We are also grateful to Sayaka Higo-Yamamoto (AIST), Dr. Tomoki Abe (University of Tokushima) and Arisa Ochi (University of Tokushima) for technical assistance.

DECLARATION OF INTEREST

The authors have no conflicts of interest to report. The authors are solely responsible for the content and writing of this paper. This study was supported by operational subsidies from AIST and Grant-in-Aid for Scientific Research (C) KAKENHI to R. Nakao (24620015) and K. Oishi (25350179) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan.

Supplementary Material available online

Supplemental Figures S1–S5 and Table S1.