Properties of an Adrenal Medullary Protein Immuno-Related to Steroid Acute Regulatory (Star) Protein

Y-C Lo Department of Reproductive & Developmental Sciences (Clinical Biochemistry), University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

Sm Mackenzie Department of Reproductive & Developmental Sciences (Clinical Biochemistry), University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

Af Howie Department of Reproductive & Developmental Sciences (Clinical Biochemistry), University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

Dk Apps Department of Biomedical Sciences, University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

Ji Mason Department of Reproductive & Developmental Sciences (Clinical Biochemistry), University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

Bc Williams Department of Medical Sciences, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK

Sd Morley Department of Reproductive & Developmental Sciences (Clinical Biochemistry), University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, EH3 9YW, UK

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Abstract

Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immuno-reactive species to have a higher molecular weight (50 kDa) compared to StAR protein (30 kDa). Subcellular fractionation localised the 50 kDa species principally to the medulla cytosol. StAR peptide antiserum binding to both the 30 kDa and 50 kDa species could be specifically competed by the peptide antigen. These data suggest that the adrenal medullary immuno-reactive species and StAR protein are distinct entities, which share some features in common.

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Properties of an Adrenal Medullary Protein Immuno-Related to Steroid Acute Regulatory (Star) Protein

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Properties of an Adrenal Medullary Protein Immuno-Related to Steroid Acute Regulatory (Star) Protein

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