A sensitive, convenient radioimmunoassay procedure which demonstrates that serum htsh is suppressed below the normal range in thyrotoxic patients

R. E. Wehraann Developmental Endocrinology Section, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205

H. A. Kubenstein Developmental Endocrinology Section, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205

B. C. Nisula Developmental Endocrinology Section, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205

Abstract

A highly sensitive radioimmunoassay procedure for the measurement of serum hTSH is described which permits delineation of the entire range of values in normal subjects (0,5-4.5 /μU/ml). The procedure Involves the concentration of hTSH from serum by adsorption to concanavalin A covalently bound to 4B Sepharose, lactoperoxidase lodination of hTSH, and disequilibrium assay conditions. This method utilizes commonly available radioimmunoassay materials and is convenient to perform. Our results with this assay show that patients with thyrotoxicosis of a variety of etiologies have serum hTSH levels suppressed well below the normal range.

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A sensitive, convenient radioimmunoassay procedure which demonstrates that serum htsh is suppressed below the normal range in thyrotoxic patients

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A sensitive, convenient radioimmunoassay procedure which demonstrates that serum htsh is suppressed below the normal range in thyrotoxic patients

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