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Abstract
A simplified microassay for the transformation of lymphocytes by phytohemagglutinin (PHA) has been developed. Whole blood cultures are treated with PHA, incubated in microtiter plate wells, precipitated on glass fiber filters, and assayed for new DNA synthesis via thymidine-2-14C uptake using a multiple sample precipitator. This method uses only 7μl of blood per culture and is sufficiently rapid to permit use on large numbers of replicate samples.