Imminogenicity of Soluble Muhine Histocompatibility Antigens

Abstract

Transplantation antigens were solubilized either from Sarcoma I (Sal) cells by aqueous autolysis, or from the spleens, lymph nodes and livers of normal A mice by aqueous autolysis followed by proteolysis with papain. The immunogenicity of these preparations was examined by their effect in modifying the rejection of Sal tumor allografts in C57BL/6 recipients and/ or by their capacity to induce the formation of cytotoxic lymphocytes in the spleens or lymph nodes of the immunized animals following a single intraperitoneal administration of the soluble antigen. Depending on the dose of antigen, and the interval between immunization and allografting, either tumor enhancement or accelerated tumor rejection was obtained. The latter was shown to be accompanied by cellular immunity, primarily in the lymph nodes of the sensitized animals; however, under certain conditions, cytotoxic lymphocytes were also detectable in the spleen.