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Immunological Communications Volume 3, 1974 - Issue 3
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Original Article

A Rapid Method for the Separation of Guinea Pig IgG1 and IgG2

W. Ma The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
,
U. Rudofsky The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
,
L. Esposito The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
,
R. Dilwith The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
,
B. Pollara The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
&
R. W. Steblay The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208
Pages 285-290 | Published online: 07 Jul 2009
 
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Abstract

Immunoelectrophoretically pure IgG1 and IgG2 were isolated from sera of guinea pigs with experimental autoimmune renal tubular disease by chromatography on QAE-Sephadex A-50 (Pharmacia Fine Chemicals, Piscataway, N.J. using a two-step elution. As judged by indirect immunofluorescence tests of the fractions, 70% of the original autoantibody activity to tubular basement membrane was recovered.

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