A Rapid Method for the Separation of Guinea Pig IgG1 and IgG2

W. Ma The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

U. Rudofsky The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

L. Esposito The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

R. Dilwith The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

B. Pollara The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

R. W. Steblay The New York State Kidney Disease Institute and The Albany Medical College, Albany, New York, 12208

Abstract

Immunoelectrophoretically pure IgG1 and IgG2 were isolated from sera of guinea pigs with experimental autoimmune renal tubular disease by chromatography on QAE-Sephadex A-50 (Pharmacia Fine Chemicals, Piscataway, N.J. using a two-step elution. As judged by indirect immunofluorescence tests of the fractions, 70% of the original autoantibody activity to tubular basement membrane was recovered.

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A Rapid Method for the Separation of Guinea Pig IgG1 and IgG2

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A Rapid Method for the Separation of Guinea Pig IgG1 and IgG2

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