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Original Article

Antiseea to Carcinoembryonic Antigen: Removal of Persistant Impurities (1)

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Pages 489-495 | Published online: 07 Jul 2009
 

Abstract

Antisera to carcinoembryonic antigen preparations frequently contained antibodies to a distinct normal serum protein(s). The latter antibodies persisted following absorptions with cross-linked human plasma proteins, normal colon, and Group 0 red cells. A simple method for removal of the contaminating antibodies involved absorptions with the perchloric acid-soluble fraction of normal serum. Excesses of the absorbant and its soluble immune complexes were then removed on anion exchange columns.

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