Lymphocyte Plasma Membranes III Carbohydrate Containing Antigens of Human Lymphocytes Bound by Antilymphocyte Sera

Abstract

Lymphocytes were labeled by the sequential application of periodate ³H-borohydride. The labeled viable cells were solubilized in 0.5% NP₄₀ and the resulting soluble material was precipitated with different rabbit anti-human lymphocyte sera (ALS). The ALS specifically bound isotope which was characterized by electrophoresis on polyacrylamide gels in SDS. Three major components were visualized, a high molecular weight (MW) component(s) (MW ∼100-200,000), a component of MW ∼48,000 and very low MW material. Differences were described in the electropherograms of lymphocytes from human thymus, tonsil, blood and cultured lines. Differences were also found in the antigenic reactivity of ALS prepared against thymus and ALS prepared against cultured human lymphocytes.