Radioimmunoassay for Human T-Cell Associated Antigens on Molt 4 Cultured Cells

Abstract

We have detected antigens associated with two malignant T-cell lines by use of a sensitive radioimmunoassay. Baboon antisera to a cultured malignant T cell line (MOLT 4F) were prepared and the γG-anti-MOLT 4F was enriched by specific adsorption onto, and elution from, MOLT 4F cells followed by repeated absorption with pools of 21 established, long-term cultured B cell lines. Purified ¹²⁵I-γG-anti-MOLT 4p preparation was tested for relative binding to autologous (MOLT 4F) and allogeneic (Sommer-RPMI 8402) T cells as well as B cells, including Sommer B cells (RPMI 8392). The degree of enrichment of antibody to malignant T cell associated antigens, expressed in terms of the ratios of the amount of globulin bound by T cells relative to the amount bound by B cells, were 6.3 and 5.0 for MOLT 4F and Sommer T cells, respectively.