Spontaneous Release of Soluble HL-A Antigens from Platelets During Conservation

A. Dautigny Laboratoire d'Immuno-Hématologie Hôpital, Saint-Louis 2, place du Docteur Fournier, 75010, Paris; Laboratoire des Protéines Université de Paris, V 45, rue des Saints-Pères, 75270, Paris Cedex 06, France

I. Bernier Laboratoire d'Immuno-Hématologie Hôpital, Saint-Louis 2, place du Docteur Fournier, 75010, Paris; Laboratoire des Protéines Université de Paris, V 45, rue des Saints-Pères, 75270, Paris Cedex 06, France

J. Colombani Laboratoire d'Immuno-Hématologie Hôpital, Saint-Louis 2, place du Docteur Fournier, 75010, Paris; Laboratoire des Protéines Université de Paris, V 45, rue des Saints-Pères, 75270, Paris Cedex 06, France

P. Jolles Laboratoire d'Immuno-Hématologie Hôpital, Saint-Louis 2, place du Docteur Fournier, 75010, Paris; Laboratoire des Protéines Université de Paris, V 45, rue des Saints-Pères, 75270, Paris Cedex 06, France

Abstract

Experiments with the aim of studying the solubilisation of HL-A antigens from blood platelets by methods which do not involve any biologically active processes (moderate, discontinuous agitation of a low concentration of platelets suspended in a saline medium, in the presence of an antiseptic; supernatants collected at frequent intervals) have shown that platelets release membrane proteins, including HL-A antigens, spontaneously. Optimal conditions for the treatment of membrane proteins have been perfected. The great stability of HL-A antigens under these conditions permits prolonged treatment. The products extracted are soluble and extremely complex. The molecular weight of the HL-A antigens is between 40,000 and 70,000.

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Spontaneous Release of Soluble HL-A Antigens from Platelets During Conservation

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Spontaneous Release of Soluble HL-A Antigens from Platelets During Conservation

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