The Use of ³H Serotonin Release from Mast Cells of the Mouse as an Assay for Mediator Liberation

J. A. Otsuki Department of Microbiology Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada

R. Grassick Department of Microbiology Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada

D. Seymour Department of Microbiology Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada

L. S. Kind Department of Microbiology Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada

Abstract

The release of ³H 5-HT from murine mast cells is shown to be a simple reproducible method for studying the activation of such cells by various agents. ³H-serotonin was taken up by peritoneal cell suspensions in vitro and was released by antigen, Concanavalin A, Forssman antiserum, anti-mouse immunoglobulin, or a polypeptide antibiotic, Cinnamycin.

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The Use of ³H Serotonin Release from Mast Cells of the Mouse as an Assay for Mediator Liberation

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The Use of 3H Serotonin Release from Mast Cells of the Mouse as an Assay for Mediator Liberation

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The Use of ³H Serotonin Release from Mast Cells of the Mouse as an Assay for Mediator Liberation