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Abstract
A comparison of three methods for the enumeration of rosette forming cells in man was done with simultaneous staining for surface immunoglobulins for the identification of B-cells. The three methods were as follows: incubation of the lymphocytes with (1) sheep red cells and 25% fetal calf serum (E-rosettes), (2) sheep red blood cells and 25% horse serum (E-rosettes), and (3) neuraminidase treated sheep red blood cells and 25% fetal calf serum (EN-rosettes). The percentage of EN-rosettes (76.4% ± 3.3%, mean ± S.D.) was significantly higher than the percentage of EH-rosettes (70.4% ± 3.6%), and E-rosettes (62.1% ± 4.1%) (P < 0.001). The percentage of B-cells were not significantly different from each other under all three conditions, and less than 1% of the immunoglobulins bearing cells could form rosettes.