12
Views
11
CrossRef citations to date
0
Altmetric
Original Article

Temperature Effects on Lymphocyte Transformation In Vitro

, &
Pages 63-80 | Published online: 07 Jul 2009
 

Abstract

Phytohemagglutinin (PHA)-induced transformation of normal rat peripheral lymphocytes has been studied at a wide range of culture temperatures (4d`C to 42d`C). Lymphocyte transformation was maximum at 37d`C while insignificant stimulation was observed between 4d`C and 30d`C. Temperatures above 37d`C produced sub-optimal transformation as measured by synthesis of DNA and protein, and appearance of lymphoblasts. Binding studies using 125I-PHA indicate that the low temperature inhibition of lymphocyte transformation could be a result of excess lectin (being available as a result of low temperature) bound to the cell surface, preventing the initiation of the molecular events associated with transformation.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.