Isolation of Human T Lymphocytes by a Negative Selection Procedure

Abstract

A single step method to isolate human T lymphocytes was achieved using a rosette technique to deplete non-T lymphocytes. Erythrocytes coated simultaneously with IgG and complement were used to form rosettes with cells bearing such receptors during a short incubation period and the resetting cells removed by gradient centrifugation. T lymphocytes were isolated (>96% T cells) without exposing to much experimental manipulations and in good recovery (mean 60%, range 40-75%).