Alloantigen-Mediated Adherence of Sat Hepatocytes to Antibody-Coated Polystyrene Dishes

Abstract

Rat alloantigens expressed on normal hepatocytes have been utilized in a cell “panning” procedure to mediate the haplotype-specific adherence of collagenase-isolated hepatocytes to antibody-coated polystyrene dishes. A polyvalent F344 anti-WF alloantiserum was prepared by immunizing F344 rats with WF tissue. The alloantibody IgG purified from the alloantiserum, when reacted with either WF or (WF x F344)F₁ hybrid rat hepatocytes, caused the adherence of these hepatocytes to bacteriological-grade polystyrene dishes coated non-specifically with a xenoantibody, rabbit anti-rat IgG. Similarly-treated hepatocytes of the F344 strain, which lack WF alloantigenic determinants, failed to be adsorbed above background level to the coated dishes.