Stimulation of the Na/K Pump in LK Sheep Erythrocytes by Immunoglobulin Fragments

Abstract

Alloimmune antiserum against the L antigen of red cells from sheep of the LK phenotype is known to stimulate by several fold active Na/K transport in LK cells. We have shown that monomeric fragments, Fab', of anti-L, as well as dimeric fragments, F(ab')2, stimulate transport to the same extent as intact anti-L Ig. Special care was taken to obtain pure fragments. Two earlier reports on the effect of immunoglobulin fragments were contradictory.