A Radio (¹¹¹In) Capillary Tube Leukocyte Adherence Inhibition Assay for the Detection of Specific Tumor-Associated Immunity

Abstract

The specific tumor-associated immune response of C3H/HeJ mice was determined at various times after subcutaneous injection with a transplantable mammary adenocarcinoma (H2712) using a radio (¹¹¹In) leukocyte adherence inhibition (LAI) assay carried out in capillary tubes. Solubilized tunor-associated antigen prepared by a single phase 1-butanol extraction of the specific tumor and other transplantable tumors of different histological origin were used in the evaluation of LAI reactivity. The assay was found to be capable of detecting a significant antitumor response before the subcutaneous tumors became detectable by palpation. The response remained significant until the tumors were greater than 20 mm in diameter.