Identification of Proliferating Lymphocyte Subpopulations in Microcultures by Surface Marker and Autoradiography

Abstract

A micromethod is described which allows subpopulation classification of proliferating (³H thymidine incorporating) cells, previously stimulated in microcultures. The technique is based on transferring 1,000 to 5,000 cells from microcultures to poly-L-lysine coated multispot slides. The cells are then stained for surface markers using the immuno-peroxidase method, combined with subsequent autoradiography.