Migration in Vitro by Blood and Exudate Neutrophils Assessed Serially During an Inflammatory Response

W. Douglas Biggar Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Charmain Barker Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Graeme Hamilton Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Linda Crawford Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Desmond Bohn Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Geraldine Kent Departments of Pediatrics, Intensive Care and Research Institute The Hospital for Sick Children and, University of Toronto, Toronto, Ontario, Canada

Abstract

Migration in vitro by blood and inflammatory neutrophils has been compared serially during an inflammatory response. Using an experimental pig model, neutrophils are isolated from the peripheral blood and from the pleural space at hourly intervals after an intrapleural challenge with zymosan activated pig serum (ZAS). Following acepromazine sedation and halothane anesthesia, blood neutrophil migration was transiently reduced. By 1 hour random and directed migration of blood neutrophils returned to normal. Directed and random migration of exudate neutrophils was markedly decreased to both a stimulus-specific (ZAS) and an unrelated (LTB₄) chemoattractant. After 3 hours, migration by exudate neutrophils was similar to migration by blood neutrophils examined in parallel. These findings emphasize the importance of performing serial evaluations of cell function during an inflammatory response.

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Migration in Vitro by Blood and Exudate Neutrophils Assessed Serially During an Inflammatory Response

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Migration in Vitro by Blood and Exudate Neutrophils Assessed Serially During an Inflammatory Response

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