Interleukin Cascade Reaction Inhibition by Supernatants of the Cultures of Antibody-Transformed Human Basal Keratinocytes

Abstract

We investigated the ability of normal human basal keratinocytes, treated with antibodies against basal keratinocytes, to produce an effect upon the activity of allogenic peripheral blood mononuclear cells. We determined the influence of basal keratinocyte culture supernatants (BKCS) upon the mononuclear cell ability for proliferation in the response to PHA and Con A, for IL 1 and IL 2 production as well as for absorption of exogenous IL 2. It was found that BKCS inhibit the lectin-dependent transformation of mononuclears and interleukin cascade reactions. The conclusion was made about the capacity of antibody-transformed basal keratinocytes for down regulation of immune-response as well as about the role of antibodies in feedback interactions between interleukin-induced antibody synthesis and interleukin production.