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Immunological Investigations
A Journal of Molecular and Cellular Immunology
Volume 18, 1989 - Issue 5
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Original Article

Measurement of Serum Interleukin-2 Activity

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Pages 713-722 | Published online: 07 Jul 2009
 

Abstract

The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56°C. No loss of recombinant interleukin-2 activity occured during heating in serum-free media. The percentage of interleukin-2 activity lost at 56°C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64.

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