Circulating Regulatory T Cells in Endometrial Cancer: A Role for Age and Menopausal Status

Abstract

Regulatory T cells (Treg) are a sub-population of T cells that suppress self-reactivity and are implicated in immune tolerance towards malignant cells. Circulating Treg cells are increased in several cancers. In endometrial cancer Treg cells have been investigated only in tumour tissues and, in contrast to some other tumours, fewer Treg cells were reported in endometrial cancer compared with benign controls. Flow cytometry was used to determine the frequency of circulating Treg cells in women undergoing hysterectomy for either endometrial cancer (n = 24) or non- cancer-related conditions (n = 21). Circulating Treg cells were more abundant in women with cancer compared to those without (4.68% vs. 3.66%, p = 0.05, Mann-Whitney test). This relationship disappeared, however, when only data from post-menopausal women were included in the analysis. Mean Treg cell frequency was 4.65% in postmenopausal women with cancer (n = 23) and 4.73% in postmenopausal controls (n = 5) (p = 0.9). In women without cancer we found that mean Treg cell frequency was higher in postmenopausal women (4.73%, n = 5) in comparison to premenopausal controls (3.33%, n = 16) (p = 0.02). These results suggest that the increased proportion of Treg cells seen in endometrial cancer patients might be, at least in part, attributed to their postmenopausal status or age.

Keywords:

• Treg cells
• Endometrial cancer
• Age
• Menopause

ACKNOWLEDGMENTS

We would like to thank Prof H. Kitchener for the ongoing support and advice. We are grateful for the support and active help we received from Godfrey Wilson, Rhona McVey, Richard Fitzmaurice, Gina Howarth, Sai Daayana and Tom Southgate. We are indebted for the generosity and kindness of all participants.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.